Myoung-Ae Choe; Gyeong Ju An

doi:10.4040/jkan.2010.40.5.611

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Original Article
Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury: Myoung-Ae Choe, Gyeong Ju An; Journal of Korean Academy of Nursing 2010;40(5):611-619.
DOI: https://doi.org/10.4040/jkan.2010.40.5.611
Published online: October 31, 2010

¹Professor, College of Nursing, Seoul National University, Seoul, Korea.

²Professor, Department of Nursing, Cheongju University, Cheongju, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea. Tel: 82-2-740-8824, Fax: 82-2-765-4103, machoe@snu.ac.kr

• Received: April 29, 2010 • Accepted: October 14, 2010

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Abstract
NOTES
REFERENCES

Abstract

Purpose
The purpose of this study was to examine the effects of exercise on muscle weight and Type I and II fiber cross-sectional area of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury.
Methods
Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The Pain+Exercise (PE) group (n=21) and the Sham+Exercise (SE) group (n=20). All rats had 28 sessions of treadmill exercise at grade 10 for 30 minutes, twice/day at 10 m/min for 14 days. Body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Muscle weight and Type I, II fiber cross-sectional area of the dissected muscles were measured.
Results
The PE group showed significant increases (p<.05), as compared to the SE group for body weight and total diet intake, muscle weight of the unaffected soleus and plantaris, and in Type I and II fiber cross-sectional area of unaffected three muscles and affected plantaris.
Conclusion
Exercise for 14 days attenuates unaffected soleus, plantaris and gastrocnemius muscle atrophy in neuropathic pain model.
Keywords: Pain; Exercise; Muscular atrophy; Muscle weight; Muscle fiber

This work was supported by Mid-career Researcher Program through NRF grant funded by the MEST (2009-0053460).

REFERENCES

1. Blundell JE, King NA. Exercise, appetite control, and energy balance. Nutrition. 2000;16:519–522.Article PubMed
2. Chapman V, Suzuki R, Dickenson AH. Electrophysiological characterization of spinal neuronal response properties in anesthetized rats after ligation of spinal nerves L5-L6. Journal of Physiology. 1998;507(Pt 3):881–894.PubMed PMC
3. Chiechio S, Copani A, Nicoletti F, Gereau RW. L-acetylcarnitine: A proposed therapeutic agent for painful peripheral neuropathies. Current Neuropharmacology. 2006;4:233–237.Article PubMed PMC
4. Choe MA, An GJ. Effects of exercise before steroid treatment on type I and type II hindlimb muscles in a rat model. Journal of Korean Academy of Nursing. 2007;37:81–90.Article PubMed PDF
5. Choe MA, An GJ. Effect of dehydroepiandrosterone on affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury. Journal of Korean Academy of Nursing. 2009;39:632–640.Article PubMed
6. Choe MA, An GJ, Lee YK, Im JH, Choi-Kwon S, Heitkemper M. Effect of inactivity and undernutrition after acute ischemic stroke in a rat hindlimb muscle model. Nursing Research. 2004;53:283–292.Article PubMed
7. Choe MA, Kim KH, An GJ, Lee KS, Choi JA. Hindlimb muscle atrophy of rat induced by neuropathic pain. Journal of Korean Biological Nursing Science. 2008;10:88–95.
8. Chung KY, Shin SW, Choi BS, Kim CH, Kim KH, Kim HK. Spinal α2 adrenoceptor and antiallodynic effect by clonidine after chronic administration of 4-methylcatechol in neuropathic rat pain model. The Korean Journal of Pain. 2008;21:179–186.Article
9. Daemen MA, Kurvers HA, Bullens PH, Slaaf DW, Freling G, Kitslaar PJ, et al. Motor denervation induces altered muscle fibre type densities and atrophy in a rat model of neuropathic pain. Neuroscience Letters. 1998;247:204–208.Article PubMed
10. Hutchinson KJ, Gomez-Pinilla F, Crowe MJ, Ying Z, Basso DM. Three exercise paradigms differentially improve sensory recovery after spinal cord contusion in rats. Brain. 2004;127:1403–1414.Article PubMed
11. Kim JY. Effects of short-term undernutrition on type I and II muscles in rat. 2005;Seoul, Seoul National University. Unpublished master's thesis.
12. Kim SH, Chung JM. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain. 1992;50:355–363.Article PubMed
13. Lee EO, Choe MA. Pain-theory and intervention. 1996;Seoul, Sinkwang.
14. Lee EO, Im NY, Park HA. Nursing/medical research and statistical analysis. 1998;3rd ed. Seoul, Soomoonsa.
15. Leem JW, Gwak YS, Chung SS, Lee KR, Yoon DM, Nam DS. Effects of NO synthase inhibitor on responsiveness of dorsal horn neurons in neuropathic pain animal model. Journal of the Korean Pain Society. 2000;13:19–30.
16. Lieu F, Power SK, Herb RA, Criswell D, Martin D, Wood C. Exercise and glucocorticoid-induced diaphragmatic myopathy. Journal of Applied Physiology. 1993;75:763–771.Article PubMed
17. Luciano CA, Pardo CA, McArthur JC. Recent developments in the HIV neuropathies. Current Opinion in Neurology. 2003;16:403–409.Article PubMed
18. Naganska E, Taraszewska A, Matyja E, Grieb P, Rafaowska J. Neuroprotective effect of erythropoietin in amyotrophic lateral sclerosis (ALS) model in vitro: Ultrastructural study. Folia Neuropathologica. 2010;48:35–44.PubMed
19. O'Leary MF, Hood DA. Effect of prior chronic contractile activity on mitochondrial function and apoptotic protein expression in denervated muscle. Journal of Applied Physiology. 2008;105:114–120.Article PubMed
20. Sakurai T, Takei M, Ogasawara J, Watanabe N, Sanpei M, Yoshida M, et al. Exercise training enhances tumor necrosis factor-α-induced expressions of anti-apoptotic genes without alterations in caspase-3 activity in rat epididymal adipocytes. Japanese Journal of Physiology. 2005;55:181–189.Article PubMed
21. Sato J, Perl ER. Adrenergic excitation of cutaneous pain receptors induced by peripheral nerve injury. Science. 1991;251:1608–1610.Article PubMed
22. Schmader KE. Epidemiology and impact on quality of life of postherpetic neuralgia and painful diabetic neuropathy. Clinical Journal of Pain. 2002;18:350–354.Article PubMed
23. Trifiletti RR. Neuroprotective effects of NG-nitro-L-arginine in focal stroke in the 7-day old rat. European Journal of Pharmacology. 1992;218:197–198.Article PubMed
24. Woolf CJ, Mannion RJ. Neuropathic pain: Aetiology, symptoms, mechanism, and management. Lancet. 1999;353:1959–1964.PubMed
25. Ziegler D, Gries FA, Spuler M, Lessmann F. Diabetic cardiovascular autonomic neuropathy multicenter study group: The epidemiology of diabetic neuropathy. Journal of diabetes and its complications. 1992;6:49–57.Article PubMed

Figure 1

Cross-section of the hindlimb muscles in Pain+Exercise and Sham+Exercise rats. The first line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The second line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The third line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. The fourth line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. Dark=Type I muscle fiber, light=Type II muscle fiber (Myosin ATPase straining, 100×).

Table 1

Body Weight and Total Diet Intake of Pain+Exercise and Sham+Exercise Rats

Table 2

Total Activity Score of Pain+Exercise and Sham+Exercise Rats

Table 3

Muscle Weight of Pain+Exercise and Sham+Exercise Rats

Table 4

Cross-Sectional Area of the Hindlimb Muscles in Pain+Exercise and Sham+Exercise Rats

Figure & Data

REFERENCES

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Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

J Korean Acad Nurs. 2010;40(5):611-619. Published online October 31, 2010

DOI: https://doi.org/10.4040/jkan.2010.40.5.611

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Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Figure 1 Cross-section of the hindlimb muscles in Pain+Exercise and Sham+Exercise rats. The first line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The second line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Pain+Exercise rats. The third line is the affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. The fourth line is the unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Sham+Exercise rats. Dark=Type I muscle fiber, light=Type II muscle fiber (Myosin ATPase straining, 100×).

Figure 1

Effects of Exercise on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Table 1

Body Weight and Total Diet Intake of Pain+Exercise and Sham+Exercise Rats

Table 2

Total Activity Score of Pain+Exercise and Sham+Exercise Rats

Table 3

Muscle Weight of Pain+Exercise and Sham+Exercise Rats

Table 4

Cross-Sectional Area of the Hindlimb Muscles in Pain+Exercise and Sham+Exercise Rats

Table 1 Body Weight and Total Diet Intake of Pain+Exercise and Sham+Exercise Rats

Table 2 Total Activity Score of Pain+Exercise and Sham+Exercise Rats

Table 3 Muscle Weight of Pain+Exercise and Sham+Exercise Rats

Table 4 Cross-Sectional Area of the Hindlimb Muscles in Pain+Exercise and Sham+Exercise Rats