Yongbum Kim; Myoung-Ae Choe

doi:10.4040/jkan.2010.40.4.580

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Original Article
Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease: Yongbum Kim, Myoung-Ae Choe; Journal of Korean Academy of Nursing 2010;40(4):580-588.
DOI: https://doi.org/10.4040/jkan.2010.40.4.580
Published online: August 31, 2010

¹Student, Bredin Institute, Edmonton, AB, Canada.

²Professor, College of Nursing, Seoul National University, Seoul, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-799, Korea. Tel: 82-2-740-8824, Fax: 82-2-765-4103, machoe@snu.ac.kr

• Received: April 19, 2010 • Accepted: August 16, 2010

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Abstract
NOTES
REFERENCES

Abstract

Purpose
The purpose of this study was to examine effects of decreased locomotor activity on mass, Type I and II fiber cross-sectional areas of ipsilateral and contralateral hindlimb muscles 21 days after establishing the Parkinson's disease rat model.
Methods
The rat model was established by direct injection of 6-hydroxydopamine (6-OHDA, 50 µg) into the left substantia nigra after stereotaxic surgery. Adult male Sprague-Dawley rats were assigned to one of two groups; the Parkinson's disease group (PD; n=17) and a sham group (S; n=8). Locomotor activity was assessed before and 21 days after the experiment. At 22 days after establishing the rat model, all rats were anesthetized and soleus and plantaris muscles were dissected from both ipsilateral and contralateral sides. The brain was dissected to identify dopaminergic neuronal death of substantia nigra in the PD group.
Results
The PD group at 21 days after establishing the Parkinson's disease rat model showed significant decrease in locomotor activity compared with the S group. Weights and Type I and II fiber cross-sectional areas of the contralateral soleus muscle of the PD group were significantly lower than those of the S group.
Conclusion
Contralateral soleus muscle atrophy occurs 21 days after establishing the Parkinson's disease rat model.
Keywords: Parkinson's disease; Rats; Hindlimb; Muscle atrophy

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (810-20090016).

This article is a part of master's thesis.

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Figure 1

Experimental design.

Figure 2

TH-specific immunohistochemical staining in substantia nigra in unilateral 6-OHDA lesioned rats.

Table 1

Preweight and Postweight between the Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 2

Total Dietary Intake between the Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 3

Locomotor Activity of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 4

Muscle Weight of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 5

Cross-Sectional Area of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Figure & Data

REFERENCES

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Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease

J Korean Acad Nurs. 2010;40(4):580-588. Published online August 31, 2010

DOI: https://doi.org/10.4040/jkan.2010.40.4.580

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Figure

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Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease

Figure 1 Experimental design.

Figure 2 TH-specific immunohistochemical staining in substantia nigra in unilateral 6-OHDA lesioned rats.

Figure 1

Figure 2

Effect of Decreased Locomotor Activity on Hindlimb Muscles in a Rat Model of Parkinson's Disease

Table 1

Preweight and Postweight between the Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 2

Total Dietary Intake between the Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 3

Locomotor Activity of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 4

Muscle Weight of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 5

Cross-Sectional Area of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 1 Preweight and Postweight between the Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 2 Total Dietary Intake between the Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 3 Locomotor Activity of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 4 Muscle Weight of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups

n=number of animals.

PD=Parkinson's disease; S=sham.

Table 5 Cross-Sectional Area of Hindlimb Muscles of Parkinson's Disease (PD) and Sham (S) Groups