Myoung-Ae Choe; Gyeong Ju An

doi:10.4040/jkan.2009.39.5.632

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Original Article
Effect of Dehydroepiandrosterone on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury: Myoung-Ae Choe, Gyeong Ju An; Journal of Korean Academy of Nursing 2009;39(5):632-640.
DOI: https://doi.org/10.4040/jkan.2009.39.5.632
Published online: October 31, 2009

¹Professor, College of Nursing The Research Institute of Nursing Science, Seoul National University, Seoul, Korea.

²Full-time Instructor, Department of Nursing, Cheongju University, Cheongju, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea. Tel: 82-2-740-8824, Fax: 82-2-766-1852, machoe@snu.ac.kr

• Received: April 10, 2009 • Accepted: July 23, 2009

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Abstract
NOTES
REFERENCES

Abstract

Purpose
The purpose of this study was to examine the effect of DHEA (Dehydroepiandrosterone) on muscle weight and Type I and II fiber cross-sectional area of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury.
Methods
Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The DHEA group (n=10) had DHEA injections daily for 14 days, and the Vehicle group (n=10) had vehicle injections daily for 14 days. Withdrawal threshold, body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected from the both hindlimbs. Body weight, food intake, activity, muscle weight and Type I, II fiber cross-sectional area of the dissected muscles were measured.
Results
The DHEA group showed significant increases (p<.05), as compared to the vehicle group for muscle weight of the unaffected plantaris, and in Type II fiber cross-sectional area of the gastrocnemius muscle. The DHEA group demonstrated a higher pain threshold than the vehicle group whereas total diet intake and activity score were not significantly different between the two groups.
Conclusion
DHEA administration for 14 days attenuates unaffected plantaris and gastrocnemius muscle atrophy.
Keywords: Pain; Dehydroepiandrosterone; Muscular atrophy; Muscle weight; Muscle fiber

This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (R01-2007-000-10573-0).

REFERENCES

1. An GJ, Choe MA. Effect of DHEA on type I and II muscles in a focal cerebral ischemia model rat. Journal of Korean Biological Nursing Science. 2002;4(2):19–42.
2. Backonja MM. Defining neuropathic pain. . Anesthesia & Analgesia. 2003;97:785–790.
3. Bondesen BA, Mills ST, Pavlath GK. The COX-2 pathway regulates growth of atrophied muscle via multiple mechanisms. American Journal of Physiology & Cell Physiology. 2006;290:C1651–C1659.Article
4. Brown GA, Vukovich MD, Sharp RL, Reifenrath TA, Parsons KA, King DS. Effect of oral DHEA on serum testosterone and adaptations to resistance training in young men. Journal of Applied Physiology. 1999;87:2274–2283.Article PubMed
5. Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. Journal of Neuroscience Methods. 1994;53:55–63.Article PubMed
6. Chapman V, Suzuki R, Dickenson AH. Electrophysiological characterization of spinal neuronal response properties in anesthetized rats after ligation of spinal nerves L5-L6. Journal of Physiology. 1998;507(Pt 3):881–894.PubMed PMC
7. Charalampopoulos I, Margioris AN, Gravanis A. Neurosteroid dehydroepiandrosterone exerts anti-apoptotic effects by membrane-mediated, integrated genomic and non-genomic pro-survival signaling pathways. Journal of Neurochemistry. 2008;107:1457–1469.Article PubMed
8. Choe MA, An GJ, Lee YK, Im JH, Choi-Kwon S, Heitkemper M. Effect of inactivity and undernutrition after acute ischemic stroke in a rat hindlimb muscle model. Nursing Research. 2004;53:283–292.Article PubMed
9. Choe MA, Kim KH, An GJ, Lee KS, Choi JA. Hindlimb muscle atrophy of rat induced by neuropathic pain. Journal of Korean Biological Nursing Science. 2008;10:88–95.
10. Demoulin C, Crielaard J, Vanderthommen M. Spinal muscle evaluation in healthy individuals and low-back-pain patients: A literature review. Joint Bone Spine. 2007;74:9–13.Article PubMed
11. Dixon WJ. Efficient analysis of experimental observations. . Annual Review of Pharmacology & Toxicology. 1980;22:441–462.
12. Gudemez E, Ozer K, Cunningham B, Sieminow K, Browne E, Siemionow M. Dehydroepiandreosterone as an enhancer of functional recovery following crush injury to rat sciatic nerve. Microsurgery. 2002;22:234–241.Article PubMed
13. Hasselgren PO, Zamir O, James JH, Fischer JE. Prostaglandin E2 does not regulate total or myofibrillar protein breakdown in incubated skeletal muscle from normal or septic rats. Biochemistry Journal. 1990;270:45–50.Article PDF
14. Kaasik A, Safiulina D, Kalda A, Zharkovsky A. Dehydroepiandrosterone with other neurosteroids preserve neuronal mitochondria from calcium overload. Journal of Steroid Biochemistry & Molecular Biology. 2003;87:97–103.Article
15. Kibaly C, Meyer L, Patte-Mensah C, Mensah-Nyagan AG. Biochemical and functional evidence for the control of pain mechanism by dehydroepiandrosterone endogenously synthesized in the spinal cord. The FASEB Journal. 2008;22:93–104.PubMed
16. Kim JY. Effects of short-term undernutrition on type I and II muscles in rat. 2005;Seoul, Seoul National University. Unpublished master's thesis.
17. Kim SH, Chung JM. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain. 1992;50:355–363.Article PubMed
18. Lee EO, Choe MA. Pain-theory and intervention. 1996;Seoul, Sin-kwang Publishing Company.
19. Leem JW, Gwak YS, Chung SS, Lee KR, Yoon DM, Nam DS. Effects of NO Synthase inhibitor on responsiveness of dorsal horn neurons in neuropathic pain animal model. Journal of the Korean Pain Society. 2000;13:19–30.
20. Long JP, Greco SC. The effect of propofol administered intravenously on appetite stimulation in dogs. Contemporary Topics in Laboratory Animal Science. 2000;39(6):43–46.
21. Luciano CA, Pardo CA, McArthur JC. Recent developments in the HIV neuropathies. Current Opinion of Neurology. 2003;16:403–409.Article
22. Mensah-Nyagan AG, Kibaly C, Schaeffer V, Venard L, Meyer L, Patte-Mensah C. Endogenous steroid production in the spinal cord and potential involvement in neuropathic pain modulation. Journal of Steroid Biochemistry and Molecular Biology. 2008;109:286–293.Article PubMed
23. Morley JE, Kraenzle D. Causes of weight loss in a community nursing home. Journal of American Geriatric Society. 1994;42:583–585.
24. O'Leary MF, Hood DA. Effect of prior chronic contractile activity on mitochondrial function and apoptotic protein expression in denervated muscle. Journal of Applied Physiology. 2008;105:114–120.PubMed
25. Sato J, Perl ER. Adrenergic excitation of cutaneous pain receptors induced by peripheral nerve injury. Science. 1991;25:1608–1610.
26. Schmader KE. Epidemiology and impact on quality of life of postherpetic neuralgia and painful diabetic neuropathy. Clinical Journal of Pain. 2002;18:350–354.PubMed
27. Taiwo YO, Levine JD. Effects of cyclooxygenae products of arachidonic acid metabolism on cutaneous nociceptive threshold in the rat. Brain Research. 1990;537:372–374.PubMed
28. Trifiletti RR. Neuroprotective effects of NG-nitro-L-arginine in focal stroke in the 7-day old rat. European Journal of Pharmacology. 1992;218:197–198.Article PubMed
29. Turinsky J. Phospholipids, prostaglandin E2, and proteolysis in denervated muscle. American Journal of Physiology. 1986;251:165–173.
30. Vielhaber A, Portenoy RK. Advances in cancer pain management. Hematology Oncology Clinics of North America. 2002;16:527–541.PubMed
31. Wojtal K, Trojnar MK, Czuczwar SJ. Endogenous neuroprotective factors: Neurosteroids. Pharmacological Reports. 2006;58:335–340.PubMed
32. Ziegler D, Gries FA, Spuler M, Lessmann F. Diabetic cardiovascular autonomic neuropathy multicenter study group. The epidemiology of diabetic neuropathy. Journal of Diabetes Complications. 1992;6:48–57.

Figure 1

Time course of changes in withdrawal threshold.

^*p<.05; Pre=pre-op.

Figure 2

Cross-section of the hindlimb muscles in DHEA and vehicle rats.

The first line is affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in DHEA rats. The second line is unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in DHEA rats. The third line is affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Vehicle rats. The fourth line is unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Vehicle rats.

Dark=Type I muscle fiber, light=Type II muscle fiber (Myosin ATPase straining, 100×).

Table 1

Body Weight and Total Diet Intake of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 2

Total Activity Score of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 3

Muscle Weight of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 4

Cross-Sectional Area of the Hindlimb Muscles in DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Figure & Data

REFERENCES

Citations

Citations to this article as recorded by

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Journal of Rehabilitation Research and Development.2016; 53(4): 499. CrossRef
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Effect of Dehydroepiandrosterone on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

J Korean Acad Nurs. 2009;39(5):632-640. Published online October 31, 2009

DOI: https://doi.org/10.4040/jkan.2009.39.5.632

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Effect of Dehydroepiandrosterone on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Figure 1 Time course of changes in withdrawal threshold. *p<.05; Pre=pre-op.

Figure 2 Cross-section of the hindlimb muscles in DHEA and vehicle rats. The first line is affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in DHEA rats. The second line is unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in DHEA rats. The third line is affected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Vehicle rats. The fourth line is unaffected soleus (left), plantaris (middle) and gastrocnemius muscle (right) in Vehicle rats. Dark=Type I muscle fiber, light=Type II muscle fiber (Myosin ATPase straining, 100×).

Figure 1

Figure 2

Effect of Dehydroepiandrosterone on Affected and Unaffected Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Table 1

Body Weight and Total Diet Intake of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 2

Total Activity Score of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 3

Muscle Weight of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 4

Cross-Sectional Area of the Hindlimb Muscles in DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 1 Body Weight and Total Diet Intake of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 2 Total Activity Score of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 3 Muscle Weight of DHEA and Vehicle Rats

DHEA=dehydroepiandrosterone; n=number of animals.

Table 4 Cross-Sectional Area of the Hindlimb Muscles in DHEA and Vehicle Rats