Myoung-Ae Choe; Gyeong-Ju An

doi:10.4040/jkan.2009.39.3.321

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Original Article
Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles: Myoung-Ae Choe, Gyeong-Ju An; Journal of Korean Academy of Nursing 2009;39(3):321-328.
DOI: https://doi.org/10.4040/jkan.2009.39.3.321
Published online: June 29, 2009

¹Professor, College of Nursing, Seoul National University, Seoul, Korea.

²Assistant Professor, Department of Nursing, Catholic University of Daegu, Daegu, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-799, Korea. Tel: 82-2-740-8824, Fax: 82-2-765-4103, machoe@snu.ac.kr

• Received: December 29, 2008 • Accepted: April 14, 2009

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Abstract
NOTES
REFERENCES

Abstract

Purpose
The purpose of this study was to determine the effect of Dehydroepiandrosterone (DHEA) administration alone or exercise combined with DHEA before steroid treatment on rat hindlimb muscles.
Methods
Male Sprague-Dawley rats were assigned to one of three groups: a steroid group (S, n=10) that had no treatment for 7 days before steroid treatment; a DHEA-steroid group (DS, n=8) that had 0.34 mmol/kg/day DHEA injection once a day for 7 days before steroid treatment and an exercise+DHEA-steroid group (EDS, n=9) that ran on the treadmill combined with 0.34 mmol/kg/day DHEA injection for 7 days before steroid treatment. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Body weight, food intake, muscle weight, myofibillar protein content and cross-sectional area of the dissected muscles were determined.
Results
The DS group showed significant increases (p<.05) as compared to the steroid group in body weight, and muscle weight of gastrocnemius muscles. The EDS group showed significant increases (p<.05) as compared to the S group in body weight, muscle weight, myofibrillar protein content, and Type II fiber cross-sectional area of soleus, plantaris and gastrocnemius muscles.
Conclusion
Exercise combined with DHEA administration before steroid treatment prevents steroid induced muscle atrophy, with exercise combined with DHEA administration being more effective than DHEA administration alone in preventing muscle atrophy.
Keywords: Steroids; Dehydroepiandrosterone; Exercise; Hindlimb; Muscular atrophy

This study was funded by the Korea Research Foundation (E00096).

REFERENCES

1. An GJ, Lee YK, Lim JH, Choi S, Choe MA. Effect of exercise during acute stage on hindlimb muscles of stroke induced rat. Journal of Korean Biological Nursing Science. 2000;2(2):9–16.
2. An GJ, Choe MA. Effect of DHEA on type I and II muscles in a focal cerebral ischemia model rat. Journal of Korean Biological Nursing Science. 2002;4(2):19–42.
3. Apostolova G, Schweizer RA, Balazs Z, Kostadinova RM, Odermatt A. Dehydroepiandrosterone inhibits the amplication of glucocorticoid action in adipose tissue. American Journal of Physiology Endocrinology and Metabolism. 2005;288:E957–E964.PubMed
4. Araneo B, Daynes R. DHEA functions as more than antiglucocorticoid in preserving immunocompetence after thermal injury. Endocrinology. 1995;136:393–401.PubMed
5. Blundell JE, King NA. Physical activity and regulation of food intake: Current evidence. Medical Science of Sports. Exercise. 1999;31:11 Suppl. 573–583.
6. Brown GA, Vukovich MD, Sharp RL, Reifenrath TA, Parsons KA, King DS. Effect of oral DHEA on serum testosterone and adaptations to resistance training in young men. Journal of Applied Physiology. 1999;87:2274–2283.Article PubMed
7. Choe MA, Choi JA, Shin GS. Effect of regular exercise during dexamethasone on the body weight, weight of hindlimb muscle and adrenal gland in young rats. Journal of Korean Academy of Nursing. 1997;27:510–519.
8. Choe MA. Effect of endurance exercise during dexamethasone treatment on the attenuation of atrophied hindlimb muscle induced by dexamethasone in rats. Journal of Korean Academy of Nursing. 1998;28:893–907.Article PDF
9. Choe MA, Shin GS, Lee EJ, An GJ. Effect of DHEA administration during dexamethasone treatment on mass of hindlimb muscles of rat. Journal of Korean Biological Nursing Science. 2001;3:63–74.
10. Choe MA, Shin GS, An GJ, Choi JA, Lee YK. Effect of regular exercise during recovery period following steroid treatment on the atrophied type II muscles induced by steroid in young rats. Journal of Korean Academy of Nursing. 2002;32:550–559.
11. Choe MA, An GJ. Effects of exercise before steroid treatment on type I and type II hindlimb muscles in a rat model. Journal of Korean Academy of Nursing. 2007;37:81–90.Article PubMed PDF
12. Dhatariya K, Bigelow ML, Nair KS. Effect of dehydroepiandrosterone replacement on insulinsensitivity and lipids in hypoadrenal women. Diabetes. 2005;54:765–769.Article PubMed PDF
13. Goldspink G. Mechanical signals, IGF-I gene splicing, and muscle adaptation. Physiology. 2005;20:232–238.Article PubMed
14. Horber FF, Hoppeler H, Scheidegger JR, Grunig BE, Frey FJ. Impact of physical-training on the ultrastructure of midthigh muscle in normal subjects and in patients treated with glucocorticoids. The Journal of Clinical Investigation. 1987;79:1181–1190.Article PubMed PMC
15. Kim YS, Kim CG. Muscle fiber types by ATPase staining method. The Research Institute of Physical Education & Sports Science. 1991;10:93–103.
16. Kostka T, Arsac LM, Patricot MC, Berthouze SE, Lacour JR, Bonnefoy M. Leg extensor power and DHEA sulfate, insulin-like growth factor-I and testosterone in healthy active elderly people. European Journal of Applied Physiology. 2000;82:83–90.Article PubMed PDF
17. Kwak CK, Kang CM, Kang HS, Song KY, Lee MS, Song SC, et al. Dehydroepiandrosterone-dependent induction of peroxisomal proliferation can be reduced by aspartyl esterification animal model. Journal of Korean Medical Science. 2000;15:533–541.PubMed PMC
18. Lapier TK. Glucocorticoid-induced muscle atrophy: The role of exercise in treatment and prevention. Journal of Cardiopulmonary Rehabilitation. 1997;17(2):76–84.Article PubMed
19. Lee SJ, McPherron AC. Myostatin and the control of skeletal muscle mass. Current Opinion Genetic Development. 1999;9:604–607.Article
20. Ma K, Mallidis C, Bhasin S, Mahabadi V, Artaza J, Gonzalez-Cadavid N, et al. Glucocorticoid-induced skeletal muscle atrophy is associated with upregulation of myostatin gene expression. American Journal of Physiolology Endocrinology & Metabolism. 2003;285:E363–E371.Article
21. Morales AJ, Haubrich RH, Hwang JY, Asakura H, Yen SS. The effect of six months treatment with a 100mg daily dose of DHEA on circulating sex steroids, body composition and muscle strength in age-advanced men and women. Journal of Clinical Endocrinology & Metabolism. 1998;78:1360–1367.
22. Park SJ, Lee MC, Kim SJ. Effect of physical exercise on experimental steroid-induced myopathy. Journal of Korean Neural Association. 1999;17:694–701.
23. Roubenoff R. Sarcopenia: Effects on body composition and function. Journal of Gerontological Biology Science. 2003;58:1012–1017.
24. Sugino M, Ohsawa N, Ito T, Ishida S, Yamasaki H, Kimura F, et al. A pilot study of dehydroepi-androsterone sulfate in myotonic dystrophy. Neurology. 1998;51:586–598.Article PubMed
25. Tsuji K, Furutama D, Tagami M, Ohsawa N. Specific binding and effects of DHEA-S on skeletal muscle cells. Life Science. 1999;65:17–26.
26. Villareal DT, Holloszy JO, Kohrt WM. Effects of DHEA replacement on bone mineral density and body composition in elderly women and men. Clinical Endocrinology. 2000;53:561–568.Article PubMed PDF
27. Villareal DT, Holloszy JO. DHEA enhances effects of weight training on muscle mass and strength in elderly women and men. American Journal of Endocrinology & Metabolism. 2006;291:E1003–E1008.Article

Figure 1

Experimental design.

^*Muscle dissection.

S=steroid; DS=DHEA-steroid; EDS=exercise+DHEA-steroid.

Figure 2

Cross-section of soleus, plantaris and gastrocnemius muscles in S (left), DS (middle) and EDS (right) rats.

The first line is soleus, the second line is plantaris, and the third line is gastrocnemius in S, DS and EDS rats. Cross-section of the soleus, the plantaris and the gastrocnemius muscles in S rats was adapted from a study by Choe & An (2007).

S=steroid; DS=DHEA-steroid; EDS=Exercise+DHEA-steroid; Dark=Type I fiber; light=Type II fiber (ATPase staining, 100×).

Table 1

Body Weight and Total Amount of Diet Intake among Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 2

Muscle Weight of Steroid (S), DHEA-steroid (DS), and Exercise+ DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 3

Myofibrillar Protein Content of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 4

Cross-Sectional Area of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Figure & Data

REFERENCES

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Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

J Korean Acad Nurs. 2009;39(3):321-328. Published online June 29, 2009

DOI: https://doi.org/10.4040/jkan.2009.39.3.321

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Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

Figure 1 Experimental design. *Muscle dissection. S=steroid; DS=DHEA-steroid; EDS=exercise+DHEA-steroid.

Figure 2 Cross-section of soleus, plantaris and gastrocnemius muscles in S (left), DS (middle) and EDS (right) rats. The first line is soleus, the second line is plantaris, and the third line is gastrocnemius in S, DS and EDS rats. Cross-section of the soleus, the plantaris and the gastrocnemius muscles in S rats was adapted from a study by Choe & An (2007). S=steroid; DS=DHEA-steroid; EDS=Exercise+DHEA-steroid; Dark=Type I fiber; light=Type II fiber (ATPase staining, 100×).

Figure 1

Figure 2

Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

Table 1

Body Weight and Total Amount of Diet Intake among Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 2

Muscle Weight of Steroid (S), DHEA-steroid (DS), and Exercise+ DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 3

Myofibrillar Protein Content of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 4

Cross-Sectional Area of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 1 Body Weight and Total Amount of Diet Intake among Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 2 Muscle Weight of Steroid (S), DHEA-steroid (DS), and Exercise+ DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 3 Myofibrillar Protein Content of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 4 Cross-Sectional Area of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats