Myoung-Ae Choe; Gyeong Ju An

doi:10.4040/jkan.2011.41.4.520

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Original Article
Effects of Nitric Oxide Synthase Inhibitor on Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury: Myoung-Ae Choe, Gyeong Ju An; Journal of Korean Academy of Nursing 2011;41(4):520-527.
DOI: https://doi.org/10.4040/jkan.2011.41.4.520
Published online: August 31, 2011

¹Professor, College of Nursing, Seoul National University, Seoul, Korea.

²Assistant Professor, Department of Nursing, Cheongju University, Cheongju, Korea.

Address reprint requests to: An, Gyeong-Ju. Department of Nursing, Cheongju University, 586 Daeseong-ro, Sangdang-gu, Cheongju 360-764, Korea. Tel: +82-43-229-8992, Fax: +82-43-229-7988, antheresa@cju.ac.kr

• Received: April 5, 2011 • Accepted: August 4, 2011

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Abstract
NOTES
REFERENCES

Abstract

Purpose
The purpose of this study was to examine effects of nitric oxide synthase (NOS) inhibitor on muscle weight and myofibrillar protein content of affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury.
Methods
Neuropathic pain was induced by ligation and cutting of the left L5 spinal nerve. Adult male Sprague-Dawley rats were randomly assigned to one of two groups: The NOSI group (n=19) had NOS inhibitor (L-NAME) injections daily for 14 days, and the Vehicle group (n=20) had vehicle injections daily for 14 days. Withdrawal threshold, body weight, food intake and activity were measured every day. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected from hindlimbs. Muscle weight and myofibrillar protein content of the dissected muscles were determined.
Results
The NOSI group showed significant increases as compared to the Vehicle group for body weight at 15 days, muscle weight and myofibrillar protein content of the unaffected soleus and gastrocnemius. The NOSI group demonstrated a higher pain threshold than the vehicle group.
Conclusion
NOSI for 14 days attenuates unaffected soleus and gastrocnemius muscle atrophy in neuropathic pain model.
Keywords: Pain; Nitric oxide synthase inhibitor; Muscular atrophy; Muscle proteins

This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (R01-2007-000-10573-0).

REFERENCES

1. American College of Sports Medicine. ACSM's advanced exercise physiology. 2006;Philadelphia, Lippincott Williams & Wilkins.
2. Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. Journal of Neuroscience Methods. 1994;53:55–63. doi: 10.1016/0165-0270(94)90144-9.Article PubMed
3. Choe MA, An GJ. Effect of dehydroepiandrosterone on affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury. Journal of Korean Academy of Nursing. 2009;39:632–640. doi: 10.4040/jkan.2009.39.5.632.Article PubMed
4. Choe MA, An GJ. Effects of exercise on affected and unaffected hindlimb muscles in rats with neuropathic pain induced by unilateral peripheral nerve injury. Journal of Korean Academy of Nursing. 2010;40:611–619. doi: 10.4040/jkan.2010.40.5.611.Article PubMed
5. Choe MA, An GJ, Lee YK, Im JH, Choi-Kwon S, Heitkemper M. Effect of inactivity and undernutrition after acute ischemic stroke in a rat hindlimb muscle model. Nursing Research. 2004;53:283–292.Article PubMed
6. Choe MA, Kim KH, An GJ, Lee KS, Choi JA. Hindlimb muscle atrophy of rat induced by neuropathic pain. Journal of Korean Biological Nursing Science. 2008;10:88–95.
7. Choe MA, Kim KH, An GJ, Lee KS, Heitkemper M. Hindlimb muscle atrophy occurs from peripheral nerve damage in a rat neuropathic pain model. Biological Research for Nursing. 2011;13:44–54. doi: 10.1177/1099800410382291.Article PubMed PDF
8. Choi JW, In JH, Kim YS, Kang YJ, Im YG, Cho SM, et al. Low dose ketamine reduces the induction of ERK1/2 and CREB signaling protein in a neuropathic pain model of rats. Korean Journal of Anesthesiology. 2009;57:210–216.Article PubMed
9. Dixon WJ. Efficient analysis of experimental observations. Annual Review of Pharmacology and Toxicology. 1980;22:441–462.
10. Han SH, Lee SC, Kim YC, Lim YJ, Sung JS. Effect of L-NAME on neuropathic pain developed after peripheral nerve injury. Korean Journal of Anesthesiology. 2002;43:494–506.Article
11. Joo MJ, Lee KJ, Shin KH, Kim JC. Nitric oxide-mediated neurotoxicity after excimer laser photorefractive keratectomy. Journal of the Korean Ophthalmological Society. 1999;40:688–698.
12. Khanna A, Cowled PA, Fitridge RA. Nitric oxide and skeletal muscle reperfusion injury: Current controversies. Journal of Surgical Research. 2005;128:98–107. doi: 10.1016/j.jss.2005.04.020.Article PubMed
13. Kibaly C, Meyer L, Patte-Mensah C, Mensah-Nyagan AG. Biochemical and functional evidence for the control of pain mechanism by dehydroepiandrosterone endogenously synthesized in the spinal cord. The FASEB Journal. 2008;22:93–104. doi: 10.1096/fj.07-8930com.PubMed
14. Kim JY. Effects of short-term undernutrition on type I and II muscles in rat. 2005;Seoul, Seoul National University. Unpublished master's thesis.
15. Kim SH, Chung JM. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain. 1992;50:355–363.Article PubMed
16. Lee KC, Choi YS, Lee HB, Park ST. Effect of NMDA receptor antagonist and NOS inhibitor on spinal sensory neurons of neuropathic pain animal model. The Korean Journal of Pain. 2002;15:13–18.
17. Leem JW, Gwak YS, Chung SS, Lee KR, Yoon DM, Nam DS. Effects of NO Synthase inhibitor on responsiveness of dorsal horn neurons in neuropathic pain animal model. Journal of the Korean Pain Society. 2000;13:19–30.
18. Long JP, Greco SC. The effect of propofol administered intravenously on appetite stimulation in dogs. Contemporary Topics in Laboratory Animal Science. 2000;39(6):43–46.
19. Martinez JA, Francis GJ, Liu WQ, Pradzinsky N, Fine J, Wilson M, et al. Intranasal delivery of insulin and a nitric oxide synthase inhibitor in an experimental model of amyotrophic lateral sclerosis. Neuroscience. 2008;157:908–925. doi: 10.1016/j.neuroscience.2008.08.073.Article PubMed
20. Morley JE, Kraenzle D. Causes of weight loss in a community nursing home. Journal of the American Geriatrics Society. 1994;42:583–585.Article PubMed
21. Pizza FX, Hernandez IJ, Tidball JG. Nitric oxide synthase inhibition reduces muscle inflammation and necrosis in modified muscle use. Journal of Leukocyte Biology. 1998;64:427–433.Article PubMed PDF
22. Qi WN, Zhang L, Chen LE, Seaber AV, Urbaniak JR. Nitric oxide involvement in reperfusion injury of denervated muscle. The Journal of Hand Surgery. 2004;29:638–645. doi: 10.1016/j.jhsa.2004.01.003.Article PubMed
23. Suzuki N, Motohashi N, Uezumi A, Fukada S, Yoshimura T, Itoyama Y, et al. NO production results in suspension-induced muscle atrophy through dislocation of neuronal NOS. The Journal of Clinical Investigation. 2007;117:2468–2476. doi: 10.1172/JCI30654.Article PubMed PMC
24. Thomas DA, Ren K, Besse D, Ruda MA, Dubner R. Application of nitric oxide synthase inhibitor L-NAME, on injured nerve attenuates neuropathy induced thermal hyperalgesia in rats. Neuroscience Letters. 1996;210:124–126. doi: 10.1016/0304-3940(96)12670-7.Article PubMed
25. Trifiletti RR. Neuroprotective effects of NG-nitro-L-arginine in focal stroke in the 7-day old rat. European Journal of Pharmacology. 1992;218:197–198. doi: 10.1016/0014-2999(92)90168-4.Article PubMed
26. Wadley GD, McConell GK. Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle. Journal of Applied Physiology. 2007;102:314–320. doi: 10.1152/japplphysiol.00549.2006.Article PubMed
27. Wang SM, Tsai HP, Huang HC, Lin JL, Liu PH. Inhibition of nitric oxide synthase promotes facial axonal regeneration following neurorrhaphy. Experimental Neurology. 2009;216:499–510.Article PubMed
28. Wang Q, Wang F, Hong G. Inhibitor of nitric oxide synthase on the denervated muscle atrophy. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006;20:805–808.PubMed
29. Yoon YW, Sung B, Chung JM. Nitric oxide mediates behavioral signs of neuropathic pain in an experimental rat model. NeuroReport. 1998;9:367–372.Article PubMed

Figure 1

Time course of changes in withdrawal threshold.

^*p<.05.

NOSI=Nitric oxide synthase inhibitor.

Table 1

Body Weight and Total Diet Intake of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 2

Mean Daily Activity Score of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 3

Muscle Weight of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 4

Myofibrillar Protein Content of the Hindlimb Muscles in NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Figure & Data

REFERENCES

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Effects of Nitric Oxide Synthase Inhibitor on Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

J Korean Acad Nurs. 2011;41(4):520-527. Published online August 31, 2011

DOI: https://doi.org/10.4040/jkan.2011.41.4.520

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Effects of Nitric Oxide Synthase Inhibitor on Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Figure 1 Time course of changes in withdrawal threshold. *p<.05. NOSI=Nitric oxide synthase inhibitor.

Figure 1

Effects of Nitric Oxide Synthase Inhibitor on Hindlimb Muscles in Rats with Neuropathic Pain Induced by Unilateral Peripheral Nerve Injury

Table 1

Body Weight and Total Diet Intake of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 2

Mean Daily Activity Score of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 3

Muscle Weight of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 4

Myofibrillar Protein Content of the Hindlimb Muscles in NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 1 Body Weight and Total Diet Intake of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 2 Mean Daily Activity Score of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 3 Muscle Weight of NOSI and Vehicle Rats

NOSI=Nitric oxide synthase inhibitor.

Table 4 Myofibrillar Protein Content of the Hindlimb Muscles in NOSI and Vehicle Rats