Myoung-Ae Choe; Gyeong-Ju An

doi:10.4040/jkan.2009.39.3.321

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Original Article
Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles: Myoung-Ae Choe, Gyeong-Ju An; Journal of Korean Academy of Nursing 2009;39(3):321-328.
DOI: https://doi.org/10.4040/jkan.2009.39.3.321
Published online: June 29, 2009

¹Professor, College of Nursing, Seoul National University, Seoul, Korea.

²Assistant Professor, Department of Nursing, Catholic University of Daegu, Daegu, Korea.

Address reprint requests to: Choe, Myoung-Ae. College of Nursing, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-799, Korea. Tel: 82-2-740-8824, Fax: 82-2-765-4103, machoe@snu.ac.kr

• Received: December 29, 2008 • Accepted: April 14, 2009

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Abstract
NOTES
REFERENCES

Abstract

Purpose
The purpose of this study was to determine the effect of Dehydroepiandrosterone (DHEA) administration alone or exercise combined with DHEA before steroid treatment on rat hindlimb muscles.
Methods
Male Sprague-Dawley rats were assigned to one of three groups: a steroid group (S, n=10) that had no treatment for 7 days before steroid treatment; a DHEA-steroid group (DS, n=8) that had 0.34 mmol/kg/day DHEA injection once a day for 7 days before steroid treatment and an exercise+DHEA-steroid group (EDS, n=9) that ran on the treadmill combined with 0.34 mmol/kg/day DHEA injection for 7 days before steroid treatment. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Body weight, food intake, muscle weight, myofibillar protein content and cross-sectional area of the dissected muscles were determined.
Results
The DS group showed significant increases (p<.05) as compared to the steroid group in body weight, and muscle weight of gastrocnemius muscles. The EDS group showed significant increases (p<.05) as compared to the S group in body weight, muscle weight, myofibrillar protein content, and Type II fiber cross-sectional area of soleus, plantaris and gastrocnemius muscles.
Conclusion
Exercise combined with DHEA administration before steroid treatment prevents steroid induced muscle atrophy, with exercise combined with DHEA administration being more effective than DHEA administration alone in preventing muscle atrophy.
Keywords: Steroids; Dehydroepiandrosterone; Exercise; Hindlimb; Muscular atrophy

This study was funded by the Korea Research Foundation (E00096).

REFERENCES

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Figure 1

Experimental design.

^*Muscle dissection.

S=steroid; DS=DHEA-steroid; EDS=exercise+DHEA-steroid.

Figure 2

Cross-section of soleus, plantaris and gastrocnemius muscles in S (left), DS (middle) and EDS (right) rats.

The first line is soleus, the second line is plantaris, and the third line is gastrocnemius in S, DS and EDS rats. Cross-section of the soleus, the plantaris and the gastrocnemius muscles in S rats was adapted from a study by Choe & An (2007).

S=steroid; DS=DHEA-steroid; EDS=Exercise+DHEA-steroid; Dark=Type I fiber; light=Type II fiber (ATPase staining, 100×).

Table 1

Body Weight and Total Amount of Diet Intake among Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 2

Muscle Weight of Steroid (S), DHEA-steroid (DS), and Exercise+ DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 3

Myofibrillar Protein Content of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 4

Cross-Sectional Area of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Figure & Data

REFERENCES

Citations

Citations to this article as recorded by

Use of Animals for Nursing Research in Korea
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Journal of Korean Biological Nursing Science.2015; 17(4): 324. CrossRef
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Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

J Korean Acad Nurs. 2009;39(3):321-328. Published online June 29, 2009

DOI: https://doi.org/10.4040/jkan.2009.39.3.321

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Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

Figure 1 Experimental design. *Muscle dissection. S=steroid; DS=DHEA-steroid; EDS=exercise+DHEA-steroid.

Figure 2 Cross-section of soleus, plantaris and gastrocnemius muscles in S (left), DS (middle) and EDS (right) rats. The first line is soleus, the second line is plantaris, and the third line is gastrocnemius in S, DS and EDS rats. Cross-section of the soleus, the plantaris and the gastrocnemius muscles in S rats was adapted from a study by Choe & An (2007). S=steroid; DS=DHEA-steroid; EDS=Exercise+DHEA-steroid; Dark=Type I fiber; light=Type II fiber (ATPase staining, 100×).

Figure 1

Figure 2

Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

Table 1

Body Weight and Total Amount of Diet Intake among Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 2

Muscle Weight of Steroid (S), DHEA-steroid (DS), and Exercise+ DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 3

Myofibrillar Protein Content of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 4

Cross-Sectional Area of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 1 Body Weight and Total Amount of Diet Intake among Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 2 Muscle Weight of Steroid (S), DHEA-steroid (DS), and Exercise+ DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 3 Myofibrillar Protein Content of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats

^*p<.05; ^†Data previously published in Choe and An (2007).

n=number of animals.

Table 4 Cross-Sectional Area of the Hindlimb Muscles in Steroid (S), DHEA-steroid (DS), and Exercise+DHEA-steroid (EDS) Rats